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Fig. 1. (A) Cricket in flexed-leg thanatosis placed on a flat wooden bar. See
Materials and methods for induction procedure. The bodily movement during
thanatosis was monitored with a photo-coupler (P) settled beside the abdomen.
(B) Catalepsy during hanging. The cricket continued to be immobile with the
femoro–tibial (F–T) joint opened (30–40°) by the body
mass until arousal. (C) Set-up for extracellular recording in a minimally
restrained cricket. A pair of insulated copper wires were inserted through
small holes on the cuticle to the flexor tibiae muscle and are bound with an
earth electrode extended from the pronotum and fixed on the forewings. Each
insertion point of the wire was fixed by wax resin (grey). (D) The recording
site, revealed by a forward-fill of the main leg nerve, nerve 5B2 (N5B2). The
recording electrode remained inserted. The leg was cleared with methyl
salicylate. This preparation, in which the tip of the recording wire contacts
with the proximal flexor nerve, was used for
Fig. 4A.
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