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Fig. 1. Schematic representation of the set-up for the measurement of the transepithelial voltage with a semi-open preparation of stomach segments of larval mosquitoes. The luminal perfusion (PL) is achieved by connection of the perfusion pipette to a push-pull multi-speed syringe pump. The direction of the perfusion can be changed by connection to the infusion syringe or the withdrawal syringe. Bath perfusion (PB) is by gravity flow from interchangeable reservoirs with oxygenated salines. The transepithelial voltage is measured with calomel electrodes connected to the pipette (VL) and the bath (VB) by agar bridges. D, drainage.





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