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Fig. 3. Effects of serotonin on apical membrane potential
(Vap). The data are plotted as
Vap so that upward shifts correspond to more
lumen-positive potentials. The left panels show representative recordings, and
the right panels show values of Vap (mean +
S.E.M.) for each of the three phases of the
response to serotonin. Addition of 106 mol
l1 serotonin is indicated by downward arrows. Addition of
105 mol l1 bumetanide is indicated by
upward arrows. Tubules were bathed in control saline (top panels; replotted
from Ianowski and O'Donnell,
2001), K+-free saline (middle panels, N=6) or
Na+-free saline (bottom panels, N=4). Different letters in
each panel denote columns that differ significantly (P<0.05;
one-way ANOVA and TukeyKramer multiple comparisons).