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Fig. 8. Respiration of gill mitochondria from sulfide-free mussels with sequential
inhibition of terminal oxidases. (A,B) Traces of oxygen partial pressure
PO2 (black line) and sulfide concentration (red
line) over time. Sulfide additions at 6.25 µmol l–1 (a),
12.5 µmol l–1 (b) and 18.75 µmol l–1
(c) were repeated under non-limiting oxygen conditions and in the presence 1
mmol l–1 potassium cyanide (KCN) and 1 mmol
l–1 salicylhydroxamic acid (SHAM) to inhibit cytochrome
c oxidase and the alternative oxidase, respectively. (C) Derivatives
of oxygen (black line) and sulfide (red line) traces showing consumption rates
(
O2 and
H2S,
respectively). Rates are lower than those from sulfide-maintained mussels
(Fig. 7) but remain coupled,
although limits are reached at all sulfide concentrations. (D) KCN-inhibition
of cytochrome c oxidase and then alternative oxidase with SHAM
results in further decreases in both oxygen and sulfide consumption rates,
however the two events remain coupled. Note the compressed time scale in B and
D.
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