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Fig. 7. Respiration of gill mitochondria from sulfide-maintained mussels with
sequential inhibition of terminal oxidases. (A,B) Traces of oxygen partial
pressure PO2 (black line) and sulfide
concentration (red line) over time. Sulfide additions at 6.25 µmol
l–1 (a), 12.5 µmol l–1 (b) and 18.75
µmol l–1 (c) were repeated under non-limiting oxygen
conditions and in the presence of 1 mmol l–1 potassium
cyanide (KCN) and 1 mmol l–1 salicylhydroxamic acid (SHAM) to
inhibit cytochrome c oxidase and the alternative oxidase,
respectively. (C) Derivatives of oxygen (black line) and sulfide (red line)
traces showing consumption rates. Sulfide consumption rate
(
H2S)
proportionally increases while oxygen consumption rate
(O2) reaches a
limit and is inhibited at the highest sulfide concentration. (D)
KCN-inhibition of cytochrome c oxidase results in truncated and
unmatched rates of oxygen and sulfide consumption. SHAM-inhibition of
alternative oxidase results in complete oxygen consumption inhibition while
sulfide consumption continues at a low level. Note the compressed time scale
in B and D.
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