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Fig. 3. Hsp70 and Hsc71 mRNA expression in hepatocytes of Trematomus bernacchii, exposed to heat and chemical stress. (A) Northern slot blots of Hsp70 mRNA in hepatocytes from four individuals (lanes 1–4) exposed for 1 h to 0–12°C or to 100 µmol l–1 CdCl2 or 100 µmol l–1 MG132. Also depicted are samples of hepatocytes exposed to DMSO controls (CdCl2 and MG132 were delivered in DMSO solutions; see Materials and methods). Samples were probed with Hsp70 mRNA probes generated in T. bernacchii. (B) Levels of Hsp70 and Hsc71 mRNA in the hepatocytes from four individuals. No significant effect of treatment on the concentration of either message was observed. Values are means ± S.E.M.





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