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Fig. 4. Myoglobin is temporally expressed during muscle differentiation. (A) Immunohistochemical localization of myoglobin in differentiated C2C12 myotubes. Note that myoglobin (green) is uniformly expressed in the cytoplasm of differentiated myotubes and absent in the nuclear compartment (propidium iodide stains red and demarcates the nuclear compartment). (B) Western blot analysis of myoglobin expression following differentiation of C2C12 myotubes. Myoglobin (Mb) and myosin heavy chain (MHC) proteins increase with exposure of C2C12 myogenic cell line to differentiation media (DM).





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