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Fig. 6. Specificity check of the urea transporter (UT) antibodies by absorption
test. (A,B) The same nephron segments were stained with antibodies raised
against the C-terminal peptide (anti-UTC) and N-terminal peptide (anti-UTN) of
the UT. Treatment of the anti-UTC antibody with the UTN peptide did not affect
immunoreactivity (C), while preabsorption with the UTC peptide resulted in
complete extinction of the immunoreactive signals (arrows in D). Scale bar,
200 µm.