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Fig. 3. (A-C) Immunohistochemical localization of the urea transporter (UT; stained in brown colour) in the Triakis kidney using antibody raised against the C-terminal peptide of UT. Sections are counterstained with haematoxylin. (A,B) Immunoreactive signal was detected in tubules in the bundle zone (BZ) but not in tubules in the sinus zone (SZ) and in renal corpuscles (RC). (C) Magnified view of five tubular segments in the bundle zone. Only one segment (the collecting tubule) was immunoreactive to UT. (D,E) Confocal laser scanning micrographs of immunoreactive tubules stained with fluorescein-labelled secondary antibody. Cross-sectional view (D) and sagittal view (E) of immunoreactive tubules. Scale bars: A, 200 µm; B, 100 µm; C-E, 20 µm.





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