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Fig. 4. (A-F) Transmission electron micrographs of Carcinus maenas epithelial gill cells in zoea IV(A), megalopa (B), juvenile crab I (C-E) and adult (F). (A) Two epithelial cells of gill buds with central nuclei surrounded by a few mitochondria. (B) Marginal tip of a posterior gill filament in megalopal stage. The development of basolateral infoldings is visible around the hemolymph lacuna, and numerous vesicles are located within the cytoplasm of the epithelial cells. (C) Apical cell part of an ionocyte with mitochondria in close contact to apical microvilli, note endocuticle detached from epidermal layer. (D) High magnification of apical microvilli showing a band desmosome connection (arrow) between two neighbouring cells. (E) Basal cell part of an ionocyte with deep basolateral infoldings of the cytoplasmic membrane. Numerous elongated mitochondria are visible in close contact with basolateral infoldings. (F) Epithelial ionocyte of a posterior gill filament with apical microvilli and numerous mitochondria in close contact with basolateral infoldings of the cytoplasmic membrane. Vesicles are noticeable at apical and basal cell poles. Bm, basal membrane; cu, cuticle; hl, hemolymph lacuna; mi, mitochondrium; mv, microvilli; nu, nucleus; ve, vesicle. Bars, 2 µm (A,B); 1 µm (C-F); 0.2 µm (D).





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