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Fig. 3. Changes in mitochondrial membrane potential (
m) in
isolated oyster hemocytes exposed to different cadmium concentrations for 72 h
as measured by TMRM staining. (A) Representative distributions of the TMRM
fluorescence intensity of control and cadmium-exposed cells before or after
treatment with a mitochondrial uncoupler (CCCP). (B) Quantitative graph of the
data shown in A indicating the average TMRM fluorescence intensity in control
cells and cells exposed to different cadmium concentrations before (-CCCP) and
after (+CCCP) exposure to the mitochondrial uncoupler CCCP. The striped bar
represents TMRM fluorescence in control hemocytes without digitonin treatment.
Vertical bars represent S.E.M.
*Values are significantly different from the control (0 µmol
l-1 Cd2+); 
values significantly
different from the fluorescence intensity of the respective cell populations
in the absence of CCCP (P<0.05; N=6-8).
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