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Fig. 2. Specificity of affinity-purified antibody Affi5. Cos-1 cells were transiently transfected with EGFP-Fxr1 (A,B) or with EGFP-Fxr2 (C,D), and stained with Affi5. The EGFP signal (A,C, green) is present in both transfected Cos-1 cells, while Affi5 labelling is only present in EGFP-Fxr1 transfected Cos-1 cells (B, red). Cos-1 cells transfected with EGFP-Fxr2 show absence of labelling after Affi5 incubation (D). Lysates of EGFP-Fxr1 (lanes 1) and EGFP-Fxr2 (lanes 2) transfected Cos-1 cells were immunoblotted using antibodies against EGFP (E, lanes 1 and 2) and Affi5 (F, lanes 1 and 2). Note the absence of cross reactive material in the lane with EGFP-Fxr2 transfected Cos-1 cells using Affi5 antibodies (F, lane 2). Bars, 10 µm.





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