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Fig. 5. Intact G. morsitans tubules. (A) Control tubules subjected to alkaline phosphatase staining protocol without chromogenic substrate; the junction with the ureter is shown with an arrow; (B) as in A but subjected to alkaline phosphatase staining protocol in the presence of NBT/BCIP chromogenic substrate; the junction with the ureter is shown with an arrow; (C) high-power magnification of B; (D) as in C but different tubule preparation showing lower tubule/ureter boundaries and normally close association of tubules at the lower segment (arrows); (E) DAPI-stained; (F) as in E but also stained for alkaline phosphatase. Lower tubule segments remain closely intertwined in tubules shown in E and F. Scale bars, 100 µm.





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