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Fig. 2. The pattern of ubiquitin immunostaining in astrocytes changes after glucose
deprivation (GD). Astrocyte cultures were subjected to sham wash (A,B) or
allowed to recover 16 h after an 8 h GD insult (CI). Uninjured cells
show diffuse immunoreactivity with greater nuclear staining compared to
cytoplasmic (A,B). After GD there is loss of nuclear staining and the
appearance of aggregates throughout the cytoplasm, varying from coarse to
fine. Scale bar, 10 µm.