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Fig. 1. Ubiquitin staining patterns in CA1 neurons, 24 h following 8 min of dense
forebrain ischemia. Ubiquitin staining is color-coded green, ß-gal
staining is color-coded red. (A) A sham control animal not subjected to
ischemia was examined 24 h later. A diffuse pattern in the processes
(arrowheads) with strong nuclear staining (arrow) is observed. (B) In an
animal subjected to dense forebrain ischemia, the pattern has changed to a
patchy pattern in processes (arrowheads) with little nuclear staining (arrow).
(C) An animal subjected to ischemia following injection with Herpes vector
encoding only ß-gal and then colabeled for ß-gal to identify a
vector targeted cell shows the same pattern in the targeted neuron as in B and
in neighboring untargeted neurons with loss of nuclear staining (arrow). (D)
An animal injected with Hsp-72 vector shows a relatively maintained pattern of
ubiquitin staining in the neuron that is overexpressing Hsp72; note
colocalization of ubiquitin and ß-gal staining in the nucleus (arrow)
identified by the yellow color.
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