|
| |
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. [pH]o neutralization prevents apoptosis. Conditioned medium was generated
as described in Fig. 2. (A) The
top panel shows DNA ladders from the cardiac myocytes used to generate the
conditioned medium. In the bottom panel, the conditioned medium was added
directly to fresh plates of cardiac myocytes (middle two lanes) or it was
neutralized to pH 7.4 with Hepes (20 mmol l–1 final
concentration) and NaOH and then added to a second set of fresh cardiac
myocytes. Both sets of plates were incubated under hypoxia for 24 h and
analyzed for DNA fragmentation. Control plates were incubated under aerobic or
hypoxic conditions in parallel. (B) Parallel sets of cardiac myocytes were
exposed to hypoxia without medium change; in the first set (left panel), the
acid was allowed to accumulate exactly as described in
Fig. 2B; in the second set
(right panel), experimentally predetermined aliquots of Hepes and NaOH were
added every 12 h to maintain a [pH]o of 
7.2.
|
