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Fig. 3. Induction of apoptosis by conditioned medium. Cultures were grown under
hypoxia with or without medium change. After 48 h, the medium was removed, and
the cells were analyzed for DNA fragmentation (top panel). The conditioned
media was centrifuged at 800 g for 5 min to pellet cells and
debris, added directly to a second set of plates, and these were incubated in
air or under hypoxia as indicated. After 24 h, these cells were also harvested
and analyzed for DNA fragmentation. [pH]o was measured in all cases
immediately before harvesting the cells. Note that the control samples shown
in the last two lanes of the bottom left panel did not receive conditioned
medium. Results are representative of three separate experiments.
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