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Fig. 8. HIF-1{alpha} accumulates in vivo in response to both hypoxic hypoxia and cobalt chloride, administered intraperitoneally, in the 6 month rat brain cortex. At 24 months, however, there is greatly diminished accumulation of HIF-1{alpha} under hypoxic conditions, but the response to cobalt chloride is still robust. ß-actin was used as a loading control (Chavez and LaManna, 2003).





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