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Fig. 3. Identification of medial hair (MH)-activated motor activity. (A) Whole
nerve cords from the thoracic to the terminal ganglion were removed along with
the attached A4 proleg. Recordings were made from nerves contralateral to the
removed proleg. The dorsal nerve (DN) was dissected such that the anterior
(DNA), lateral (DNL) and posterior (DNP)
branches were distinct, and extracellular recordings were made from each
branch. The ventral nerve (VN) was not dissected into separate branches.
Representative traces are shown of the spike activity before, during and after
MH stimulation, which is indicated by the marker trace below each record. (B)
Histograms showing mean responses of each nerve branch to an MH stimulus. The
number of distinct events was counted for 1 s before the stimulus (open bars)
and for 1 s beginning 1 s after the start of an MH stimulus (shaded bars).
Each bar is the mean (± S.E.M.) of 3-6
preparations. Asterisks indicate significant differences (paired
t-tests, P<0.05).