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Fig. 3. (Top) Northern blot analysis of plasma membrane Ca2+-ATPase (PMCA3) in various crayfish tissues in intermolt (A), premolt (B) and postmolt (C). Total RNA (20 µg) from gill, antennal gland, cardiac muscle and axial abdominal muscle was loaded in each lane. The membrane was hybridized to a 1164 bp crayfish PMCA3 probe and exposed to X-ray film for 24 h. (Bottom) To normalize the hybridization signal, 18s rRNA concentration was run on a corresponding formaldehyde-agarose gel and visualized by ethidium bromide staining under UV light before being transferred to the membrane. The band size is indicated to the left.





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