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Fig. 1. (A) Nucleotide sequence of open reading frame (ORF) and deduced amino acid sequence of crayfish antennal gland plasma membrane Ca2+-ATPase (PMCA3). Nucleotides and amino acids are numbered on the right. The putative start codon ATG and stop codon TAG are in bold. Sequences corresponding to the primers used in both initial PCR and 5'/3' RACE are underlined. (B) Comparison of the deduced amino acid sequence of crayfish antennal gland plasma membrane Ca2+-ATPase (PMCA3) with that of human PMCA3a (GenBank accession No. U57931), tilapia PMCA2 (AF23669) and bullfrog PMCA2a (AF337956). Stars below amino acids indicate identity between residues in PMCA from different species. Amino acids are numbered on the right. Transmembrane regions are underlined and labeled TM1-TM10. The phosphorylation site, fluorescein isothiocyanate (FITC) site (assumed to be part of the ATP-binding site), the 5'-p-fluorosulphonylbenzoyladenosine/r-(N-2-chloroethyl-N-methylamino) benzylamine ATP-binding (FSBA) site, calmodulin (CaM) binding site and PMCA3 Ab peptides location are underlined in both A and B. This sequence has been accepted by GenBank (accession number AY455931).





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