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Fig. 1. Nucleotide and deduced amino acid sequence of the PrL opsin of Pieris rapae crucivora. Single- or double-headed arrows indicate the regions corresponding to primer attachment: the sequences of degenerate primers are TTGAAGCTTCARTTYCCNCCNATGAAYCC (L01F, forward primer for QFPPLNP), TTGAAGCTTAYGTNCCIGARGGNAAYATGAC (L02F, forward primer for PEGNMTA), CGAATTCGTCATRTTNCCYTCIGGNACRTA (L02R, reverse primer for PEGNMTA) and CGAATTCGGRTTRTANACIGCRTTNGCYTT (L03R, reverse primer for KANAVYNP). Forward and reverse primers have a HindIII site and EcoRI site (underlined), respectively.





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