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Fig. 7. Gradation between S1 and S2 muscle phenotypes
identified through SDS-PAGE and western blotting, demonstrating the
correlation among MHC, TnT and TnI isoforms. Predominantly S2
muscle samples are on the left (lanes a,b), a predominantly S1
sample is on the right (h), and samples following a gradation from
S2 to S1 are in the middle (c-g) (a,b,h are single
fibers, while c-g are fiber bundles). Myosin heavy chains (MHC) were
identified by SDS-PAGE and silver staining. The S2 MHC isoform has
a lower electrophoretic mobility and is identified by a filled circle. The
S1 MHC has a higher mobility and is identified by an open circle.
The four samples on the left (a-d) only possessed the S2 MHC, the
middle samples (e-g) had both S1 and S2 isoforms, and
the last sample (h) possessed only the S1 isoform. Troponin (Tn) T
and I isoforms were identified by SDS-PAGE followed by western blotting. The
TnT isoforms showed an orderly progression from TnT1 (a,b) to both
TnT1,3 (c-g) to only TnT3. The TnI isoforms showed a
progression from TnI2 (a,b) to TnI2,3 (c-e), to
TnI3 (f-h). Various proportions of TnI1 and
TnI4 were also observed in several of the samples (d,e,g,h).
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