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Fig. 2. Effects of 10–4 mol l–1 bumetanide on (A) fluid secretion, (B) K+ flux and (C) Na+ flux for tubules after exposure to ouabain. After exposure to 10–4 mol l–1 ouabain for 30 min, bumetanide was added to Malpighian tubules in the experimental group (filled bars) and the vehicle (0.1% ethanol) was added to the control group (open bars). Data are expressed as mean ± S.E.M. Asterisks indicate significant differences between flux before and after addition of bumetanide (paired t-test, P<0.05, N=11).





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