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Fig. 7. Comparison of staining techniques visualizing the IPS (arrows) in 3 h post-molt exocuticle. (A) Immunohistochemistry using the antibody against the Edman degradation-derived amino acid sequence (anti-peptide). This image has been digitally enhanced to increase the contrast between the `prisms' and the IPS. (B) Toluidine Blue staining showing that the IPS may actually have a higher concentration of total proteins than the prisms, especially near the epicuticle boundary.





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