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Fig. 6. Sections of 0 h (A–C), 1 h (D–F), 2 h (G–I) and 3 h
(J–L) post-molt cuticle probed with antibodies raised against either the
purified glycoprotein (A,D,G,J) or a 15-amino-acid peptide determined by Edman
degradation of the purified material (B,E,H,K). Detection was by
fluorescent-labeled secondary antibodies. Controls (C,F,I,L) lacking primary
antibody show some autofluorescence in the epicuticle (arrowhead) and the
hypodermal cells but none in the exocuticle (bracket). All photos were taken
at the same light intensity and exposure time and were not digitally altered.
Arrows indicate the interprismatic septa (IPS) staining less intensely at the
later time periods. Scale bars, 50 µm.
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