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Fig. 5. Colocalization of synapsin-enriched structures with serotonergic and
dopaminergic fibres. Whole-mount preparations of salivary glands were
triple-labelled with anti-synapsin (red), BODIPY-FL phallacidin (blue), and
either anti-serotonin (green) or anti-TH (green). (B–D,H–L) Series
of confocal images through acinar tissue, each image representing the sum of 6
consecutive optical sections (inter-section distance: 0.4 µm). (A,G) The
sum of all images. ASP foci on the outer surface of the acinar tissue
colocalize with either anti-serotonin (B–D) or anti-TH (H–J). ASP
foci lie deep in the acinar tissue, amongst C-cells that are identified by
short phallotoxin-labelled microvilli (arrowheads) on their luminal surface,
and colocalize with anti-serotonin only (arrows in F,L). (M,N) Within the
nerves that interlink adjacent acinar lobules, most ASP foci (arrows)
colocalize with anti-TH; few foci colocalize with anti-serotonin (insets in
M). (O,P) ASP foci on initial duct segments colocalize with either
anti-serotonin or anti-TH. (Q,R) On the large salivary ducts, ASP foci
colocalize with anti-TH almost exclusively. (Insets in P,R) Horizontal
sections through a duct at the position indicated by the dotted boxes in (P
and R) demonstrate the distribution of ASP foci along dopaminergic fibres
within the duct epithelium. Broad arrows indicate autofluorescent tracheoles.
Scale bars, 10 µm (A–L); 25 µm (M–R).
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