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Fig. 3. Immunolocalization of synapsin in serotonergic nerve fibres at the release
sites for neurotransmitters or neurohormones. (A–C) Nerve fibres in a
cockroach muscle double-stained with anti-serotonin and anti-synapsin.
Anti-synapsin immunoreactivity is highly concentrated at the sides of the
nerve terminals (arrowheads in C). (D–F) A salivary duct nerve costained
with anti-serotonin and anti-synapsin. The salivary duct nerve contains two
thick, centrally localized axons that are non-serotonergic and homogeneously
stained with anti-synapsin (E, arrows). These axons are surrounded by several
thin serotonergic axons that ramify and have numerous short sidebranches
forming a neurohaemal organ. Anti-synapsin is highly concentrated at the
distal ends of these sidebranches (arrowheads in F). Bars, 50 µm.
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