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Fig. 2. Cross-reactivity of anti-Drosophila-synapsin with cockroach
synapsin. (A) Western blot analysis of Drosophila head (lane 1),
cockroach brain (lane 2) and cockroach salivary gland (lane 3) with
anti-synapsin. A broad band with an electrophoretic mobility of about 80 kDa
is intensely labelled in all preparations. In Drosophila, an
additional synapsin isoform is detected at about 140 kDa. (B) In
vitro binding of the synapsin-positive cockroach protein to actin
filaments. An extract of cockroach brain was incubated with F-actin; actin
filaments were then pelleted by high-speed centrifugation. In the absence of
F-actin, the anti-synapsin-positive proteins remain entirely in the
supernatant (S), whereas in the presence of F-actin, a substantial fraction of
the anti-synapsin-positive proteins is detected in the pellet (P) together
with F-actin. The actin-binding protein 
-spectrin provides a positive
control, and tubulin a negative control.
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