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Fig. 1. Measurement of mitochondrial ROS production using homovanillic acid (HVA) as fluorophore in a peroxidase-catalysed reaction. Data interval, 0.02·min. M, start of measurement by addition of mitochondrial isolate to the buffer solution; 1, basal fluorescence of HVA suspension; HRP, addition of horseradish peroxidase; S, sodium succinate addition induces state 2; ADP addition induces state 3; `break' indicates the change of slope caused by the transition to state 4 after complete phosphorylation of ADP; O, oligomycin addition starts state 4+; dotted arrow, calibration with H2O2.





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