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Fig. 2. Azan Heidenhains stain of sections from stage D control zooids (A,C,E), compared to 6 mg BHT l-1-treated zooids (B,D,F) after 3 days arrested at stage D. (A) A control ramet with two resorbed zooids at stage D. (B) BHT-treated zooid after 3 days arrested at stage D. Internal organs, such as endostyle, branchial sac and siphon, are intact. (C) Enlargement of the deteriorating endostyle area of a control zooid. (D) Enlargement of the BHT-treated zooid endostyle area with unspoiled structures including morphologically normal epithelial cells. (E) Stage D 22 control, the deteriorated zooid branchial sac stigmata area. Leftover cells and macrophages carrying debris are seen. (F) Third day arrested stage D zooid, branchial sac stigmata area. The organ is intact. z, zooid; bs, branchial sac; en, endostyle; s, siphon; st, stigmata; mac, macrophage. Scale bars, 5 µm.





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