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Fig. 1. Control B. schlosseri ramet (A,C,E) and a 6 mg BHT
l-1-treated ramet (B,D,F). (A) Control ramet in stage D. The zooid
resorption has already started and fully developed buds ready for the takeover
stage are visible; (B) BHT-treated ramet after 5 days under BHT
administration, reaching the first day of blastogenic stage D; (C) control
ramet 1 day later, already at blastogenic stage A. The old generation of
zooids was completely resorbed. (D) The corresponding BHT-treated ramet, 1 day
later (6th day of BHT exposure). The old gene ration of zooids in the center
still exists. The new generation of zooids dispersed in the tunic matrix
without the typical patterning of systems. (E) The control ramet 1 day later,
at normal blastogenic development, already at stage B; (F) the BHT-treated
ramet after 7 days of BHT exposure. The old generation of zooids was not
resorbed, while the new generation of zooids developed abnormal round shapes
of zooids with open, inhalant siphons and accumulated deep pigmentation. The
tunic looks opaque. z, zooid; z (b), new generation zooid; b, bud; am,
ampulla; t, tunic. Scale bars, 1 mm.
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