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Fig. 6. Confocal images of 40 µm sections showing RFamide immunoreactivity
(green, Cy2) and synaptotagmin immunoreactivity (red, Cy5) in antennal lobes
(ALs) after hemolymph injection of 20E or after injection of the vehicle alone
at stage P1 and dissection 3 days later at pupal stage P4. In A and B,
additional Normarski images are shown (gray). (A) After injection of the
vehicle alone ALs were indistinguishable from ALs of untreated animals at
stage P4. In the lateral cell group (broken outline) two cell bodies are
labeled. (B) 3 days after 20E injection the number of RFamide-ir cells in the
LC (broken outline) increased to levels normally observed about 2 days later
(see Fig. 5A). The glomerular
pattern reached a stage normally observed at pupal stage P7/8
(Dubuque et al., 2001). Arrows
point to developing glomeruli. (C) Posterior section through an AL showing a
dense meshwork of strongly RFamide-ir arborizations, which is normally not
observed in P4 or any other pupal stage. Frontal sections; scale bars, 80
µm (A,B); 40 µm(C). M, medial; D, dorsal.
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