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Fig. 4. Developmental time course of numbers of RFamide-ir cell bodies in the
lateral cell group. Acquisition of RFamide immunostaining in AL neurons occurs
in two phases. A first increase from stage P5 to P7/8 results in about 25
cells. The second increase in cell number (up to about 60 cells) occurs from
P11/12 to P16. Each data point indicates mean ± S.D. The
number of antennal lobes is indicated at each stage; stages P0+P1 and P2+P3
were pooled. The three cells at stages P0 to P4 are the type II and III
neurons. The dotted line indicates adult eclosion at stage P20. Inset shows
hemolymph concentrations of 20E during the pupal–adult transition as
reported by Warren and Gilbert
(1986). The open and filled
large arrows mark the times of 20E injection at P1, P9 (asterisk) and P10/11,
respectively, the open and filled circles (asterisk after P9 injection) mark
the times when the injected animals were killed and processed for
immunocytochemistry (see Fig.
5).
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