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Fig. 8. Gyc-88E and Gyc-89Db are expressed in neurons of the
peripheral nervous system. In situ hybridization (blue/black stain)
was combined with immunohistochemical staining with the neuronal antibody
22C10 (brown stain). Stained cells are indicated with open arrowheads and are
identified where possible. (A,B) Expression of Gyc-88E and
Gyc-89Db in the lateral neuron clusters of segments T2 and T3. The
example shown is segment T3 (T2 was identical). Gyc-88E (A) and
Gyc-89Db (B) were expressed in one of the three les neurons that
innervate a lateral external basiconical sensillum. In both of these panels,
dendrites from the three les neurons can be observed to extend towards the
basiconical sensillum (location marked with an asterisk). (C,D) Expression of
Gyc-88E and Gyc-89Db in the ventral neuron clusters of
segments T2 and T3. Gyc-88E (C) and Gyc-89Db (D) were
expressed in one of the three ves neurons that innervate a ventral external
basiconical sensillum. Again, the example shown is in segment T3 (T2 was
identical). (E,F) Expression of Gyc-88E and Gyc-89Db in the
neuron clusters of segments A1 and A2. Gyc-88E (E) and
Gyc-89Db (F) were expressed in one of two v'td that innervate
specific tracheal branches. The example shown is in segment A1 (A2 was
identical but the staining was less intense). The stained cell was always the
most anterior of the pair. (G–I) Expression of Gyc-88E and
Gyc-89Db in the head segment. Gyc-89Db was expressed in
4–5 neurons in each of the dorsal ganglia (one on each side) (G), and in
2–5 neurons in each of the terminal (maxillary) ganglia (one on each
side) (H). Gyc-88E expression was found in one neuron in each
terminal ganglion (I). Note the dendrites projecting towards the head
sensilla. (J,K) Expression of Gyc-88E and Gyc-89Db in the
caudal sensory cones in the telson. Gyc-88E (J) and Gyc-89Db
(K) were expressed in one of at least two neurons that innervate each of the
caudal sensory cones in segment A9. The neighboring neuron that does not
express guanylyl cyclase is indicated with an asterisk. In K, the dendrite is
clearly seen to extend from the neuron that expressed Gyc-89Db to the
extreme tip of the sensory cone, appearing to extend past the edge of the main
body of the cone as a short protrusion. Scale bar, 30 µm.
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