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Fig. 7. Localization of Gyc-88E and Gyc-89Db in cells associated
with the embryonic peripheral nervous system. In situ hybridization
on stage 17 embryos also revealed that Gyc-88E or Gyc-89Db
were both expressed in several peripherally located cells. (A,C) Expression of
Gyc-88E. (B,D) Expression of Gyc-89Db. A and B show the
horizontal view, while C and D show the lateral view. On each side of the
embryo, two cells were detected in segments T1, T2 and T3, arranged in either
an upper row or a lower row of cells, and a single cell was detected in A1 and
A2. Left is always anterior and down is ventral in side views. (E) Application
of both probes simultaneously resulted in the same number of cells staining in
segments T1–A2 as when each probe was applied individually. (F,G)
Expression of Gyc-88E and Gyc-89Db in the head segment.
Gyc-88E (F) was expressed in a pair of cells, while Gyc-89Db
(G) was expressed in more cells (2–5 per side in the clusters in focus,
and 4–5 per side in clusters located in a more posterior position and
out of focus, indicated with open arrowheads). Horizontal views are shown, and
anterior is up. (H–J) Expression of Gyc-88E and
Gyc-89Db in segments A8 and A9. Three consecutive focal planes of
focus are shown starting from most ventral (left) to dorsal (right) to capture
all cells. Both Gyc-88E (H) and Gyc-89Db (I) are expressed
in a total of 12 cells. The cells are numbered arbitrarily. (J) When both
probes were used simultaneously, the number of cells detected remained the
same. Scale bar, 200 µm.
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