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Fig. 6. Gyc-88E and Gyc-89Db expression in the central nervous
system of third instar larvae. In situ hybridization was performed on
isolated third-instar central nervous systems using the same riboprobes used
in the embryo experiments. (A,B) Expression in the brain lobes.
Gyc-88E (A) and Gyc-89Db (B) expression was found in
scattered cells throughout the brain but was most prominent in a small cluster
of cells located in the anterior medial region of each brain lobe (open
arrowheads). (C–F) Expression in the ventral nerve cord.
Gyc-88E (C,E) and Gyc-89Db (D,F) expression was found in
scattered cells located laterally and in the midline (midline marked with
broken line). The pair of images shown in C and E and in D and F are from the
same preparation but are viewed at different focal planes. Anterior is up in
all panels. Scale bar, 200 µm.
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