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Fig. 3. Expression of Gyc-88E in larvae and adults. (A) Northern blot
showing the 6 kb transcript for Gyc-88E is present in both larvae (L)
and adults (A). For a loading control, membranes were stripped and
re-hybridized with a DIG-labeled riboprobe for the ribosomal protein RP49. (B)
Both splice variants of Gyc-88E are expressed in both larvae and
adults. RT-PCR was used to amplify across the junction between exons 10 and 11
to distinguish between Gyc-88E-S and Gyc-88E-L. Both a 70 bp
(Gyc-88E-S) and a 91 bp band (Gyc-88E-L) were detected in
samples from both larvae and adults in the presence of reverse transcriptase
(+). These two bands were not observed when the reverse transcriptase was
omitted (–). The 140 bp band observed in all lanes results from the
amplification of Gyc-88E genomic DNA contamination.
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