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Fig. 7. Radial oxygen profiles in eggs of Manduca sexta. A calibrated
Clark-style microelectrode was advanced through a small hole in the egg
chorion (egg surface=0 µm) and then in 100 µm increments. The two bottom
traces are 3-day-old eggs (at 24 and 37°C, N=7 and 9,
respectively), the middle two traces are freshly laid eggs (laid in previous
12 h, N=4 and 3 at low and high temperatures, respectively), and the
top two traces are 2- and 3-day-old traces of dead eggs (killed by freezing in
liquid N2 or in a –80°C freezer, N=2 and 4,
respectively). Initial measurements were from still water (temperature
equilibrated and air saturated). Values are means ± S.E.M.
The dead 2-day-old eggs do not have error bars because N=2 eggs for
this treatment.
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