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Camera’s eye view of developing eggs of Manduca sexta
Each dot is a single egg in a well that separates it from its neighbors but exposes it to the gas stream flowing through the channel. Each of the eight channels contains a different mixture of oxygen in nitrogen (number written on right side), which each of the 10 eggs in a channel experiences. The software sampling the video stream wrote an image to a computer hard drive every 10 min. The movie shows, in time lapse, how survival (hatching) and development time depend on oxygen availability – those exposed to the most oxygen developed fastest and hatched most reliably. At this temperature (22°C), not all eggs hatched at 9 and 11·kPa oxygen. This same set-up was replicated simultaneously across three temperatures (22, 27 and 32°C).
Files in this Data Supplement:
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