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Fig. 1. Natural and laboratory temperatures experienced by adult Austrofundulus
limnaeus. (A) Temperature record for 5 consecutive days in a typical
ephemeral pond inhabited by A. limnaeus
(Podrabsky et al., 1998). (B)
Adult male fish were exposed to four different thermal acclimations. For the
cycling temperature (20–37°C) treatment (black line) fish were
collected (open circles) every 4 h for the first three daily cycles and then
again every 4 h on day 5 and day 14. Control fish (gray line) kept at 26°C
were collected (open squares) every 4 h on day 1 and day 14. Fish exposed to
constant 20°C (blue line) were collected (open blue diamonds) at 24, 48,
72, 96, 168 and 336 h. Fish exposed to constant 37°C (pink line) were
collected (open pink squares) as the 20°C fish, except for the 336 h time
point. Time 0 for the temperature cycling is 12:30 h at a temperature of
26°C. Note the broken axis in B. While five temperature cycles were
sampled during the 2-week acclimation, the temperature was continually
cycling.
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