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Fig. 3. (A) Western blot detection of ERK1 protein in the testicular extracts of
R. esculenta at various times, 4°C and 8 h:16 h photoperiod.
Proteins (25 mg/lane) were resolved by SDS-PAGE, transferred to nitrocellulose
membranes and then incubated with antibody raised against phospho-ERK1 and
ERK1 protein. A specific band was observed of 44 kDa (determined by comparison
with comigrating size markers). (B) The amount of activated ERK1 was
quantitated by using ImageQuant 5.2 Program and normalized by total ERK1. The
values shown are representative of three separate experiments.
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