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Fig. 7. SpC3 binds to yeast surfaces. Coelomic fluid was obtained from sea urchins
that had received two injections of LPS at 2-day intervals and was collected 2
days after the second injection. Yeast were incubated with ASW or CF, and SpC3
was detected on the yeast surface with anti-SpC3
'pep (primary;
1°) and G RIgAP (secondary; 2°) followed by substrate
incubation in 4-nitrophenol phosphate prior to absorbance readings at 415 nm.
Yeast+ASW, yeast were incubated with ASW but antibodies were omitted prior to
analysis; yeast+CF, yeast were opsonized with CF but antibodies were omitted
prior to analysis; yeast+CF+2°, yeast were opsonized with CF but only the
2° antibody was used prior to analysis; yeast+CF+1°+2°, yeast were
opsonized with CF and incubated with 1° and 2° antibodies prior to
analysis; yeast+1°+2°, yeast were incubated with ASW and 1° and
2° antibodies were added prior to analysis. Values are means ±
S.E.M.(N=3).
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