spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Opsonic activities are influenced by SpC3 and blocked by anti-SpC3-6H. Coelomic fluid (CF) was collected from sea urchins that had received two injections at 2-day intervals of LPS and was harvested 1 day after the second LPS injection. Yeast were opsonized for 40 min and phagocytosis was allowed to proceed for 45 min. Yeast were opsonized with either CF (yeast+CF), CF incubated for 2 h with anti-SpC3-6H (1:20 dilution; yeast+CF+{alpha}-SpC3-6H), CF incubated for 2 h with anti-profilin (1:20 dilution; yeast+CF+{alpha}-profilin) or ASW (yeast+ASW). Some of the yeast that had been opsonized with CF+anti-SpC3-6H were acid-washed prior to use in phagocytosis (yeast+CF+{alpha}-SpC3-6H washed). PSI, phagocytic stimulation index. Values are means ± S.E.M. (N=6). *P<0.05, yeast+CF+{alpha}-SpC3-6H vs. yeast+CF.





Right arrow Return to article