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Fig. 4. Opsonic activities of coelomic fluid (CF; number of yeast phagocytosed per
100 coelomocytes) plotted against the SpC3 concentration of CF (relative
intensities) collected from the same sea urchin. Sea urchins (N=18)
were injected with either LPS or ASW on day 0 and day 4. Coelomic fluid was
collected on day 1, day 1 or day 5. SpC3 titers were determined by
densitometric analysis of western blots on which SpC3 was stained with
anti-SpC3-6H. Values are means ±
S.E.M.(N=3).