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Fig. 8. Effect of L-NAME (N
–nitro-l-arginine methyl ester) on
contractile force by steroid-treated ventricle strips. Ventricle strips from
both male (A) and female (B) rainbow trout were treated with L-NAME (1.0 mmol
l–1) for 10 min prior to exposure to 11-ketotestosterone
(11KT; 0.3 µmol l–1), testosterone (T; 0.3 µmol
l–1), cortisol (C; 0.1 µmol l–1 in males
and 0.01 µmol l–1 in females) or estradiol (E2; 1.0 nmol
l–1). L-NAME had no independent effect on contractile force;
however, L-NAME limited inotropic effects of androgens, C and E2. All values
are means + S.E.M. (N=6–8).
*P<0.05 versus EtOH control strips.
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