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Fig. 4. Effects of multiple steroids on maximum contractile force developed by
ventricle strips from rainbow trout at 0.5 Hz. Ventricle strips from both male
(A) and female (B) rainbow trout were treated with 11-ketotestosterone (11KT;
0.3 µmol l–1), testosterone (T; 0.3 µmol
l–1), cortisol (C; 0.1 µmol l–1 in males
and 0.01 µmol l–1 in females), estradiol (E2; 1.0 nmol
l–1) or combinations of two different steroids at the same
concentrations. In males, the maximal effects of 11KT+C (178±4%) and
T+C (163±3%) were completely additive when compared with their
independent effects (11KT, 143±2%; T, 136±5%; C, 132±5%).
By contrast, maximal effects of E2 (129±4%) and C (136±4%) were
not additive (143±3%). All values are means +
S.E.M. (N=9). *P<0.01
compared with strips treated with one steroid hormone.
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