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Fig. 6. Effect of dietary P restriction on rate of Pi uptake in the proximal
intestine (open bars) and pyloric caeca (PC; filled bars) of rainbow trout.
Fish were fed either low-P (LP) or high-P (HP) diet for 20 days. Sleeves of
intestine and PC were sampled from the fish at days 2, 5 and 20, and Pi-uptake
analyzed in vitro. Values are means ± S.E.M.
(N=5 fish, 2 tissue sleeves per fish assayed). The tissue incubation
medium contained 0.1 mmol l–1 Pi. At this Pi concentration,
the active component represents 
75% of total Pi uptake in PC (see
Fig. 3). Between LP and HP
fish, Pi-uptake was not different at days 2 and 5 in intestine and PC;
however, at day 20, the uptake was markedly different between LP and HP fish
in the intestine (*P=0.002), but not in PC (NS; P=0.35).
Among sampling days (day 2, 5 and 20) in each treatment, Pi uptake was
markedly different in the intestine (ANOVA P=0.001 and 0.03,
regression P=0.0003 and 0.007 in LP and HP fish, respectively), but
not in PC (ANOVA P=0.34 and 0.95, regression P=0.14 and 0.76
in LP and HP fish, respectively).
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