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Fig. 4. Effect of pH on the total uptake of Pi in pyloric caeca of rainbow trout.
Values are means ± S.E.M. (N=5). *At the same pH,
Pi uptake in Na-containing (Na+) medium was significantly greater than that in
Na-free (Na–) medium (two-tailed t-test). The incubation medium
contained 0.1 mmol l–1 Pi, and estimated active Pi uptake was
75% of the total uptake. In Na-free medium, choline chloride replaced
NaCl of the normal medium iso-osmotically. The pH of the medium was adjusted
using HCl and KOH. A single buffer system (Tris-citrate) was used for all the
incubation media. Pi uptake increased at high pH in Na+ medium, but not in
Na-free medium. Significant Pi uptake was seen in Na-free medium at pH
4–9.
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