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Fig. 1. Experimental steps and time course in series 1 and 2 for ventricular muscle
isolated from turtle and trout. Preparations were either left to rest or
electrically paced to isometric twitch force development at the rate
indicated. A representative recording of twitch force development of a turtle
preparation is included. In series 1, preparations were sampled for
biochemical determinations at the times indicated. In series 2, twitch force,
oxygen consumption and hypoxic lactate production were recorded during 30 min
of oxygenation and then during 30 min of severe hypoxia.
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